Autoimmune Disease Models

IBD models: 

 

Inflammatory bowel disease (IBD) is a chronic condition that affects the digestive system. The two main types of IBD are Crohn’s disease and ulcerative colitis. Both conditions involve chronic inflammation of the digestive tract, which can lead to a range of symptoms such as diarrhea, abdominal pain, and weight loss. The exact cause of IBD is not fully understood, but it is thought to be related to a combination of genetic, microbiota in the intestine, the integrity of the intestinal epithelial barrier, and immunological factors. 

 

Numerous models have been developed to study the biology of IBD. For instance, transferring CD4+CD45Rbhi T cells or injecting anti-CD40 antibody into immune-compromised mice can induce inflammation in the large intestine, which mimics Crohn’s disease. Similarly, administering DSS in drinking water to wild-type mice can cause a break in the intestinal epithelial barrier, resulting in an ulcerative-type colitis. Furthermore, the injection of anti-CD3 antibody can lead to a transient and recoverable inflammation in the small intestine, resembling that of celiac disease. Our company provides specialized expertise in these models and related services to other companies.

IBD Models For Efficacy Study

Name of Cell Lines Mice Used Description
CD4+CD45Rbhi T Cell Induction

Rag-/- mice

 Inflammation in large intestine. Transfer of CD4+CD45Rbhi naïve T cells but not other cells into Rag-/- mice induced a Crohn’s disease like inflammation in the colon. This model can be used to evaluate the effect of drugs on the function of T cells and myeloid cells in inflammation.

Anti-CD40 Induction

 Rag-/- mice Inflammation in large intestine. Agonist antibody against CD40 can induce infiltration of macrophages into the colon, inducing IBD in Rag-/- mice, mimicking the function of CD40-L (CD154) elevated in activated CD4+ T cells while significantly reduced the complexity of the involvement of CD4+ T cells. 

DSS Induction

 WT mice Inflammation in large intestine. DSS induction model. This model employs dextran sodium sulfate (DSS) to induce epithelial damage in the large intestine, thus mimicking the ulcerative disease in human. 

Anti-CD3 Induction

 WT mice Inflammation in small intestine. Anti-CD3 induction model. Anti-CD3 induced a transient but reversible inflammation in the small intestine, with the accumulation of activated abTCR CD8ab into the intraepithelial compartment.