Custom CAR-Macrophage Cell Development: Macrophages are critical effectors of the innate immune system, which are responsible for engulfing debris and pathogens. Recently, it has been attracting significant interest on whether macrophages could be harnessed to ‘eat’ cancer cells in tumor, in which large number of cancer cells undergo constant proliferation, thus accumulating mutations, as well as apoptosis. However, as a type of terminally differentiated adhering cells, the process of CAR-Mø cells is significantly different from that of CAR-T cells. We will provide service on induction of macrophages from its monocytic ancestors, lentiviral or retroviral transduction of macrophages, CAR-mediated phagocytosis and cytokine secretion by CAR-Mø cells, transendothelial migration, etc upon request.

References:

1. Morrissey MA, Williamson AP, Steinbach AM. et al. Chimeric antigen receptors that trigger phagocytosis. eLife. 2018; 7: e36688.
2. Klichinsky M, Ruella M, Shestova O, et al. Human chimeric antigen receptor macrophages for cancer immunotherapy. Nat Biotechnol. 2020; 38(8): 947-953.