Hybridoma Antibody Sanger Sequencing: RNA extracted from in vitro expanded single antigen-specific B cell clone or hybridoma will be converted to cDNA, followed by PCR amplification, DNA subcloning, and Sanger sequencing.
| mAb Sequencing Choice | Key Deliverables | Quality Guarantees | EDT | Price ($) |
Shopping Cart |
| Cat #42001 cDNA provided by customer |
Sequences of IgH & Igk/l pair of each B cell clone. | TBD | 1 month | Quote | |
| Cat #42002 Hybridoma provided by customer |
Sequences of IgH & Igk/l pair of each B cell clone. | TBD | 1 month | Quote | |
| Cat #42003 Antigen-specific memory B cells expanded by Partner |
Sequences of IgH & Igk/l pair of each B cell clone. | TBD | 2 months | Quote |
Sanger Sequencing of Antibody from hybridoma:
References:
- Sanger F, and Coulson AR. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 1975, 94 (3): 441–8.
- Deng Y-M, Spirason N, Iannello P. et al. A simplified Sanger sequencing method for full genome sequencing of multiple subtypes of human influenza A viruses. Journal of Clinical Virology. 2015, 68: 43–48.