RNA is notoriously unstable due to the sensitivity of the molecule to the virtually omnipresent ribonucleases (RNases). However, several measures can be used to improve the stability of mRNA, thus increase the expression of target proteins by the mRNA sequence. 1) a “cap,” i.e., a 7-methyl-guanosine residue joined to the 5′-end via a 5′-5′ triphosphate, 2) a poly(A) tail. 3) incorporation of methylated nucleotides to the mRNA. 4) Introduction of 5’ and 3’ untranslated region (UTR) sequence flanking the ORF of the gene. 4) Removing the destabilizing sequence, such as AU-rich elements and miRNA binding sites.

We provide first-class services from mRNA sequence design and synthesis, mRNA encapsulation with lipid nanoparticle, in vitro and in vivo RNA expression analysis, evaluation the activation of different Toll-Like Receptors (TLRs) by mRNA-LNP complex.